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Nucleic acid hybridization

    '''Hybridization''' is the process of combining complementary single-stranded nucleic acids into a single molecule Nucleotides will bind to their complement under normal conditions so two perfectly complementary strands will bind to each other readily Conversely due to the different geometries of the nucleotides a single inconsistency between the two strands will prevent them from binding The process can be reversed by heating the molecule
    Experimental Procedure
    Double-stranded DNA helix is heated
    Complementary strands separate
    Sample of single strands is then mixedwith another sample of single strands
    Combined sample is then cooled slowly forDNA to reanneal and form a new "hybridized"DNA molecule
    the speed at which hybridization occurs isindicative of genomic similarity between thetwo samples; percentage genomic similarity andspeed of hybridization being directly proportional
    Through processes of DNA Hybridzation analysis the relative differences and similarities between species can be mapped based on a DNA Difference Score
    Molecular techniques such as FISH and Southern blots use hybridization probes to identify RNA or DNA


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