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Sequencing by hybridization

    '''Sequencing by Hybridization''' is a class of methods for determining the order in which nucleotides occur on a strand of DNA Typically used for looking for small changes relative to a known DNA sequence The binding of one strand of DNA to its complementary strand in the DNA double-helix (aka hybridization) is sensitive to even single-base mismatches when the hybrid region is short or is specialized mismatch detection proteins are present This is exploited in a variety of ways most notable via DNA chips or microarrays with thousands to billions of synthetic oligonucleotides found in a genome of interest plus many known variations or even all possible single-base variations
    This technology has largely been displaced by Sequencing-by-synthesis based methods

    Examples of commercial systems


    References

    • Drmanac R et al. Sequencing by hybridization (SBH): advantages achievements and opportunities Adv Biochem Eng Biotechnol 2002;77:75-101 PMID 12227738
    • Hanna GJ et al. Comparison of Sequencing by Hybridization and Cycle Sequencing for Genotyping of Human Immunodeficiency Virus Type 1 Reverse Transcriptase J Clin Microbiol 2000 July; 38(7): 2715–2721 PMID 10878069

    • Church George M. Genomes for all Scientific American 2006 Jan;294(1):52 PMID 16468433

    See also

    • Sequencing by ligation